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Near-Field Scanning Optical Microscopy Advantages and Disadvantages

This article explains about Near-Field Scanning Optical Microscopy Advantages and Disadvantages and Safety Concern.

Near-Field Scanning Optical Microscopy Advantages and Disadvantages

Advantages of Near-Field Scanning Optical Microscopy

The advantages of the NSOM are given below.

  • High resolution up to 2-5nm can be obtained.
  • By contrast mechanism, the properties such as refractive index, chemical structure, and local stress can be observed.
  • The conductive, non-conductive or transparent sample can be used to examine no special sample creation needed.
  • There are advantages related to the optical microscopy and there are benefits of albeit higher resolution.
  • The benefits of the electron microscopy can be obtained through using NSOM.
  • Using NSOM also requires minimal sample preparation.
  • NSOM if operated in ambient, there can be advantages of the liquid environments (Hsu, 2001).
  • The spectroscopy techniques can be concerned in NSOM.
  • NSOM focuses to enable the probe different materials properties
  • NSOM microscope if operated effectively, there can be advantages of spectroscopy.
  • NSOM also support conventional optical microscopes.
  • NSOM focus on the light source and collection mode.
  • Nanometer resolution is concerned by NSOM.
  • Single molecule sensitivity is there in the NSOM.

Disadvantages of Near-Field Scanning Optical Microscopy

Disadvantages of NSOM:

  • There is some limitation of the NSOM are given below.
  • For the high resolution of the image, it takes a long scan time to process it.
  • The shear force mode and the other contact operations are not helpful for soft materials.
  • The working distance is very low and has the extremely shallow depth of field.
  • NSOM only limited to the surface studies.
  • Disadvantages of NSOM device include the topographic image, as there could be issues.
  • There could be correlate structural issues in the NSOM device.
  • Physical properties of NSOM device can create problems.
  • The NSOM device can be opposite sometime.
  • There could be issues of side’s reflection mode (Hsu, 2001).
  • The contact tasks are not useful for delicate materials.
  • The working separation can be low.
  • NSOM device may has tapered optical fiber.
  • In NSOM device laser light can be coupled.
  • NSOM device may had ineffective illumination source (Hsu, 2001).
  • There can be high determination of the picture.

Safety Concern of Near-Field Scanning Optical Microscopy

Safety concerns of NSOM:

  • There are some precautions to use NSOM device.
  • Cells or tissue can be damaged with the laser light while using NSOM for cell or tissue imaging.
  • Laser light can affect the operator of NSOM device.
  • Effective practices of NSOM; result in the position resolution and accuracy.
  • For NSOM scanning optical microscope; MCL-NSOM need to be used (Hsu, 2001).
  • NSOM gadget are very few to utilize.
  • Laser light can be harmful as there are tissue imaging.
  • NSOM need to be concerned with the microscope’s versatile design.
  • Constant force feedback and shear force feedback both can be effective approaches.
  • NSOM versatile designs can help researchers to focus on the instruments that are effective.
  • Feedback mechanisms is important to achieve high resolution in the tasks of NSOM
  • NSOM is helpful in the optical microscopy techniques (Hsu, 2001).
  • Feedback mechanisms need to be concerned timely.
Reference
  • Hsu, J. W. (2001). Near-field scanning optical microscopy studies of electronic and photonic materials and devices. Materials Science and Engineering: R: Reports, 33(1), 1-50.

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