GenBank: J01566.1
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LOCUS CE1CG13 6646 bp DNA circular BCT 23-JUL-2016
DEFINITION Escherichia coli plasmid ColE1, complete sequence.
ACCESSION J01566
VERSION J01566.1
KEYWORDS .
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1008 to 1370)
AUTHORS Tomizawa,J.I., Ohmori,H. and Bird,R.E.
TITLE Origin of replication of colicin E1 plasmid DNA
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 74 (5), 1865-1869 (1977)
PUBMED 325558
REFERENCE 2 (bases 1410 to 1599)
AUTHORS Bastia,D.
TITLE Determination of restriction sites and the nucleotide sequence surrounding the relaxation site of ColE1
JOURNAL J. Mol. Biol. 124 (4), 601-639 (1978)
PUBMED 712849
REFERENCE 3 (bases 1 to 1613; 6577 to 6646)
AUTHORS Oka,A., Nomura,N., Morita,M., Sugisaki,H., Sugimoto,K. and Takanami,M.
TITLE Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity
JOURNAL Mol. Gen. Genet. 172 (2), 151-159 (1979)
PUBMED 384144
REFERENCE 4 (bases 1 to 192; 384 to 519; 6644 to 6646)
AUTHORS Patient,R.K.
TITLE Characterization of in vitro transcription initiation and termination sites in Col E1 DNA
JOURNAL Nucleic Acids Res. 6 (8), 2647-2665 (1979)
PUBMED 379827
REFERENCE 5 (bases 623 to 832)
AUTHORS Morita,M. and Oka,A.
TITLE The structure of a transcriptional unit on colicin E1 plasmid
JOURNAL Eur. J. Biochem. 97 (2), 435-443 (1979)
PUBMED 380993
REFERENCE 6 (bases 4783 to 5353)
AUTHORS Ebina,Y., Kishi,F., Miki,T., Kagamiyama,H., Nakazawa,T. and Nakazawa,A.
TITLE The nucleotide sequence surrounding the promoter region of colicin E1 gene
JOURNAL Gene 15 (2-3), 119-126 (1981)
PUBMED 6271636
REFERENCE 7 (bases 639 to 753)
AUTHORS Tomizawa,J.
TITLE Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript
JOURNAL Cell 38 (3), 861-870 (1984)
PUBMED 6207934
REFERENCE 8 (bases 1 to 6646)
AUTHORS Chan,P.T., Ohmori,H., Tomizawa,J. and Lebowitz,J.
TITLE Nucleotide sequence and gene organization of ColE1 DNA
JOURNAL J. Biol. Chem. 260 (15), 8925-8935 (1985)
PUBMED 2991225
REFERENCE 9 (bases 5046 to 5110)
AUTHORS Parker,R.C.
TITLE Mitomycin C-induced bidirectional transcription from the colicin E1 promoter region in plasmid ColE1
JOURNAL Biochim. Biophys. Acta 868 (1), 39-44 (1986)
PUBMED 3092859
REFERENCE 10 (bases 57 to 123)
AUTHORS Zhang,S.P., Yan,L.F. and Zubay,G.
TITLE Regulation of gene expression in plasmid ColE1: delayed expression of the kil gene
JOURNAL J. Bacteriol. 170 (12), 5460-5467 (1988)
PUBMED 3142845
REFERENCE 11 (sites)
AUTHORS Stirling,C.J., Szatmari,G., Stewart,G., Smith,M.C. and Sherratt,D.J.
TITLE The arginine repressor is essential for plasmid-stabilizing site-specific recombination at the ColE1 cer locus
JOURNAL EMBO J. 7 (13), 4389-4395 (1988)
PUBMED 3149585
REFERENCE 12 (sites)
AUTHORS Inoue,N. and Uchida,H.
TITLE Transcription and initiation of ColE1 DNA replication in Escherichia coli K-12
JOURNAL J. Bacteriol. 173 (3), 1208-1214 (1991)
PUBMED 1846858
COMMENT The L (light) strand is shown. This sequence was kindly supplied over computer network by J.Lebowitz (04-APR-1985). The open reading frames noted by [8] are named in FEATURES by the molecular mass of their predicted products or by the names given by [8].
FEATURES Location/Qualifiers
source 1..6646
/organism=”Escherichia coli”
/mol_type=”genomic DNA”
/db_xref=”taxon:562″
/clone=”pEW2762 and pMM1″
/plasmid=”ColE1″
mRNA join (5056..6646,1..>585)
/product=”colE1-kil mRNA”
/citation= [8]
CDS join (5140..6646,1..62)
/codon_start=1
/transl_table=11
/product=”colicin E1 protein (cea)”
/protein_id=”AAB59121.1″
/translation=”METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSK
SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEAL
RHNASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEI
EREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSR
LSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRR
RVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLK
KAQNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAA
FEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSD
ILKIKDTGDWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYI
DKNKLNTINEVLGI”
mRNA complement (<59..409)
/note=”imm mRNA [5], [8]”
CDS complement (59.400)
/codon_start=1
/transl_table=11
/product=”colicin E1 immunity protein (imm)”
/protein_id=”AAB59122.1″
/translation=”MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIV
ISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
LLGLFISIKNN”
misc_difference 83.84
/note=”conflict”
/citation= [4]
/replace=””
misc_difference 99.100
/note=”conflict”
/citation= [4]
/replace=””
misc_difference 135
/note=”conflict; replace 133..133 in Nucleic Acids Res. 6,
2647-2665 (1979)”
/citation= [4]
/replace=””
misc_difference 144
/note=”conflict; replace 142..142 in Nucleic Acids Res. 6,
2647-2665 (1979)”
/citation= [4]
/replace=””
misc_difference 174
/note=”conflict; replace 172..172 in Nucleic Acids Res. 6,
2647-2665 (1979)”
/citation=[4]
/replace=””
CDS 448..585
/codon_start=1
/transl_table=11
/product=”kil protein”
/protein_id=”AAB59123.1″
/translation=”MRKRFFVGIFAINLLVGCQANYIPDVQGGTIAPSSSSKLTGIAV
Q”
misc_difference 484..485
/note=”conflict; replace 101 in Nucleic Acids Res. 6,
2647-2665 (1979)”
/citation= [4]
/replace=””
misc_difference 491..492
/note=”conflict; replace 107 in Nucleic Acids Res. 6,
2647-2665 (1979)”
/citation= [4]
/replace=””
misc_difference 506..512
/note=”conflict; replace 121 in Nucleic Acids Res. 6,
2647-2665 (1979)”
/citation= [4]
/replace=””
misc_difference 517..519
/note=”conflict; replace 121..131 in Nucleic Acids Res. 6,
2647-2665 (1979)”
/citation= [4]
/replace=””
misc_difference 528
/note=”conflict; replace 528 in J. Biol. Chem. 260,
8925-8935 (1985)”
/citation=[8]
/replace=””
CDS 605..895
/citation= [8]
/codon_start=1
/transl_table=11
/product=”10.2 kd ORF”
/protein_id=”AAB59124.1″
/translation=”MRSFFSARNLLLANKKTTATNGGLFAGSRATNSFSEGNWLQQSA
DTKYCPSSVAVVGPLLQELCSTVCAIIALLIRLPVAAASGVKACLTGLDSRR”
misc_RNA 641.>641
/note=”II RNA”
misc_RNA complement (645.751)
/note=”I RNA ((3′ end +/- 1 bp) [3]”
misc_difference 652.653
/note=”conflict; replace 31.32 in Eur. J. Biochem. 97,
435-443 (1979)”
/citation= [5]
/replace=””
ORIGIN
1 ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
61 aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
121 aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
181 ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
241 ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
301 ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
361 ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
421 ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
481 ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
541 gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
661 taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
721 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
781 acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
841 ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
961 aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
1021 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
1081 gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
1141 ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
1201 ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
1261 cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
1321 gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc
1381 attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa
1441 tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt
1501 cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct
1561 cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
1621 ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
1681 aagcgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag
1741 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
1801 cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc
1861 gagaggatgc tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt
1921 gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt
1981 tccggaaaac tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat
2041 aacctgaatc agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt
2101 caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggctgtc
2161 agggaacagg gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag
2221 tggtccggtt gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca
2281 gggtataccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac
2341 gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat
2401 ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg
2461 ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct
2521 acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc
2581 ttggcagacg ctgttaaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg
2641 caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt
2701 cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat
2761 tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat
2821 tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt
2881 tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa
2941 tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg
3001 agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa
3061 aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg
3121 cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg
3181 ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg
3241 aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga
3301 acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga
3361 aagagtgggt acatctgaag gagtgacaca gagtgacaga gctggaaaca catttgctga
3421 acgccttaga gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct
3481 tcgtggaatt gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc
3541 gggtcatgca gttgagtcag caggtgcaac acttgagcga gcagacagaa cgcttgagcc
3601 agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgt
3661 gttatcccta gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggcccttc
3721 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
3781 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgc
3841 agtttttagg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
3901 gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
3961 gccatcgaga taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag
4021 gtctttgctg cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa
4081 ttcttggctg tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc
4141 tttgtcttta gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt
4201 gtcggctttt tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg
4261 ccgggtatgt tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt
4321 tatcatggcg ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta
4381 aagtatttat tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc
4441 gttcagcgac cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc
4501 ctgagctggg ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc
4561 atatcctgtg cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagt
4621 tcccggagaa tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc
4681 ctttctggtg tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca
4741 aactcccgtc gtccgtccct gccggtacgg taactgacaa ggcctccgcg catatcacgg
4801 taaaactgtg aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc
4861 ataaagtgac agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt
4921 ctgagaacgg taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt
4981 gaaagactgt ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa
5041 atgcagcggc gtagctttta tgctgtatat aaaaccagtg gttatatgta cagtatttat
5101 ttttaactta ttgttttaaa agtcaaagag gattttataa tggaaaccgc ggtagcgtac
5161 tataaagatg gtgttcctta tgatgataag ggacaggtaa ttattactct tttgaatggt
5221 actcctgacg ggagtggctc tggcggcgga ggtggaaaag gaggcagtaa aagtgaaagt
5281 tctgcagcta ttcatgcaac tgctaaatgg tctactgctc aattaaagaa aacacaggca
5341 gagcaggctg cccgggcaaa agctgcagcg gaagcacagg cgaaagcaaa ggcaaacagg
5401 gatgcgctga ctcagcgcct gaaggatatc gtgaatgagg ctcttcgtca caatgcctca
5461 cgtacgcctt cagcaacaga gcttgctcat gctaataatg cagctatgca ggcggaagca
5521 gagcgtttgc gccttgcgaa agcagaagaa aaagcccgta aagaagcgga agcagcagaa
5581 aaggcttttc aggaagcaga acaacgacgt aaagagattg aacgggagaa ggctgaaaca
5641 gaacgccagt tgaaactggc tgaagctgaa gagaaacgac tggctgcatt gagtgaagaa
5701 gctaaagctg ttgagatcgc ccaaaaaaaa ctttctgctg cacaatctga agtggtgaaa
5761 atggatggag agattaagac tctcaattct cgtttaagct ccagtatcca tgcccgtgat
5821 gcagaaatga aaacgctcgc tggaaaacga aatgaactgg ctcaggcatc cgctaaatat
5881 aaagaactgg atgagctggt caaaaaacta tcaccaagag ccaatgatcc gcttcagaac
5941 cgtccttttt ttgaagcaac cagacgacgg gttggggccg gtaagattag agaagaaaaa
6001 caaaaacagg taacagcatc agaaacacgt attaaccgga taaatgctga tataactcag
6061 atccagaagg ctatttctca ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat
6121 gaagctgaag aaaatttgaa aaaagcacag aataatctcc ttaattcaca gattaaggat
6181 gctgttgatg caacagttag cttttatcaa acgctgactg aaaaatatgg tgaaaaatat
6241 tcgaaaatgg cacaggaact tgctgataag tctaaaggta agaaaatcgg caatgtgaat
6301 gaagctctcg ctgcttttga aaaatacaag gatgttttaa ataagaaatt cagcaaagcc
6361 gatcgtgatg ctatttttaa tgcgttggca tcggtgaagt atgatgactg ggctaaacat
6421 ttagatcagt ttgccaagta cttgaagatt acggggcatg tttcttttgg atatgatgtg
6481 gtatctgata tcctaaaaat taaggataca ggtgactgga agccactatt tcttacatta
6541 gagaagaaag ctgcagatgc aggggtgagt tatgttgttg ctttactttt tagcttgctt
6601 gctggaacta cattaggtat ttggggtatt gctattgtta caggaa